CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

1957年，Puck TT從成年中國(guó)倉(cāng)鼠卵巢的活檢組織建立了CHO細(xì)胞，CHO-K1是CHO的一個(gè)亞克隆。CHO-K1的生長(zhǎng)需要

培養(yǎng)條件：F12K培養(yǎng)基(SIGMA,貨號(hào)N3520，添加NaHCO3 2.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%

動(dòng)物種別：中國(guó)倉(cāng)鼠

性別：雌

組織來源：卵巢

形態(tài)：上皮細(xì)胞

傳代方法的介紹   1：4-1：8

支原體檢測(cè)   陰性

以下是此細(xì)胞ATCC

CHO-K1 (ATCC® CCL-61™)
CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

Organism Cricetulus griseus, hamster, Chinese

Tissue  ovary

Product Format  frozen

Morphology  epithelial-like

Culture Properties  adherent

Biosafety Level  1

Gender  female

Applications

This cell line is suitable as a transfection host.

Storage Conditions  liquid nitrogen vapor phase

Karyotype  Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.

Derivation

The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Clinical Data

female

Virus Susceptibility  Vesicular stomatitis, Orsay (Indiana)

Vesicular stomatitis, Glasgow (Indiana)

Getah virus

Virus Resistance

poliovirus 2; modoc virus; Button Willow virus

Comments

The cells require proline in the medium for growth.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Once or twice between subculture

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C