CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

ATCC® Number: CRL-2149™ Price: $338.00

Designations: SK-N-DZ

Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow

Cell Type: neuroblast;

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Tumorigenic: Yes

DNA Profile （STR）: Amelogenin: X

CSF1PO: 12

D13S317: 8,11

D16S539: 9,11

D5S818: 12

D7S820: 12,13

THO1: 6,9

TPOX: 8

vWA: 16,18

Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region （HSR）; no double minutes were seen

Age: 2 years

Gender: female

Ethnicity: Caucasian

Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.

Retinoic acid induces differentiation in this line.

Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.

Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.

The cells exhibit moderate MDR1 expression.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694

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